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Transient receptor potential vanilloid member 1 (TRPV1) is a Ca2+-permeable cation channel that serves as the primary heat and capsaicin sensor in humans. In published work, we determined the structures of apo and capsaicin-bound fulllength rat TRPV1 reconstituted into lipid...

CRISPR-associated transposition systems allow guide RNA-directed integration of a single DNA cargo in one orientation at a fixed distance from a programmable target sequence. In published work, we defined the mechanism explaining this process by characterizing the transposition regulator, TnsC...

Bacterial pathogens are a threat to global health and have evolved elaborate strategies to infect their hosts, including the deployment of type IV secretion systems (T4SS). These membrane-embedded macromolecular machines transport proteins and DNA across multiple membranes to deliver cargo...

The ability to fight a viral infection is essential for all organisms. Drosophila melanogaster utilizes RNA interference for the antiviral response. Here, the protein, Dicer-2, is used for small interfering RNA (siRNA) biogenesis and passing one strand of the siRNA...

CryoEM has revolutionized structural studies of membrane proteins over the last decade. Combined with membrane mimetics such as lipid nanodiscs, cryoEM allows for detergent-free structural determination of membrane proteins within a lipid bilayer more closely matching the native environment. I...

My group is interested in structure, function and dynamics of pentameric ligand-gated ion channels (pLGICs). The pLGICs are central players in mediating fast synaptic transmission in the neuronal- and neuromuscular junctions. My talk will focus on two members of the...

Tips & tricks of note: Sample preparation: Testing detergents and nanodisks for integral membrane proteins. Data collection: Optimizing pixel size and data collection rate. Data processing: Using symmetry during 3D reconstruction; cleaning particle stacks with 3D classification.

Using Cryo-EM to investigate the assembly, structures and functions of polymeric antibodies Immunoglobulin (Ig) heavy chain classes, including IgA and IgM in mammals, birds and reptiles, and IgM and IgT in teleost fish, can be assembled into polymeric antibodies of...