The bacterial pathogen/host inteface: Cutting up the host

Tips & tricks of note:

Sample preparation: Using NMR analysis of point-mutants to choose an ideal cryoEM sample. Ideas for overcoming preferred orientation.

Data processing: Understanding substrate specificity by solving multiple structures. 
 

Alternative methods: Site-directed mutagenesis, NMR as a complementary technique, RMSD analysis of substrate bound/unbound structures.