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Tips & tricks of note: Sample preparation: Testing detergents and nanodisks for integral membrane proteins. Data collection: Optimizing pixel size and data collection rate. Data processing: Using symmetry during 3D reconstruction; cleaning particle stacks with 3D classification.

Using Cryo-EM to investigate the assembly, structures and functions of polymeric antibodies Immunoglobulin (Ig) heavy chain classes, including IgA and IgM in mammals, birds and reptiles, and IgM and IgT in teleost fish, can be assembled into polymeric antibodies of...