Tips & tricks of note: Sample preparation: Using NMR analysis of point-mutants to choose an ideal cryoEM sample. Ideas for overcoming preferred orientation. Data processing: Understanding substrate specificity by solving multiple structures. Alternative methods: Site-directed mutagenesis, NMR as a...
Electron cryotomography of cells: progress and potential: Grant Jensen, Professor, Brigham Young University; Electrochemical Liquid TEM of Active Soft Matter: Joe Patterson, Assistant Professor, University of California, Irvine
DNA polymerase ζ (Polζ) belongs to the same B-family as high-fidelity replicative polymerases, yet is specialized for the extension reaction in translesion DNA synthesis (TLS). Despite its importance in TLS, the structure of Polζ was unknown. We resolved structures of...
The sense of hearing and balance begins with the mechanosensory transduction (MT) channel, which converts mechanical stimuli into electrochemical signals, and which is typically localized within hair cells of the mammalian inner ear. Despite decades of effort focused on discovering...
Cutting-edge technologies and methods for in situ structural biology with cryo-ET: Juergen Plitzko, Group Leader, Max Planck Institute Germany; Grain boundary and Surface Atomic Structures and Diffusion in Li-Ion Battery Related Crystals: Yuchi Ikuhara, Professor, University of Tokyo.
Data processing: Practicalities of neural network picker software installation and usage. How to choose a particle picker & validation. Neural network picking for single particle analysis, filaments, & tomography. Interplay of particle picking and preferred orientations. Particle centering.
Genomic regions with high guanine content have the capacity to fold into four-stranded non-B DNA structures known as G-quadruplexes (G4s). Extensive bioinformatic inquiries have revealed that G4 motifs are conserved and non-randomly distributed throughout the genome. G4s are epigenetic features...
Understanding the Invisible Hands of Sample Preparation for Cryo-EM: Peter J. Peters, Professor, Maastricht University, The Maastricht Multimodal Molecular Imaging Intstitute (M4I) Using Extremely Large 4D-STEM Experiments to Measure Materials Properties and Solve 3D Structures at Atomic Resolution: Staff Scienist...
